ISOLATION AND IDENTIFICATION OF COMMON PATHOGENIC BACTERIA FROM GUT OF HONEY BEE (APIS MELLIFERA)

Abstract

The study focused on isolating and identifying the species of bacteria that might have possible relations with the pathogenesis in the digestive tract of the honey bees (Apis mellifera) and determining their susceptibility patterns of antibiotics in the Sanghr district. To attain the objectives, a cross sectional type of study was used. Forty pooled samples of live adult honey bee were taken. The frequency and percentages of the occurrence of bacterial pathogens were described using summary of descriptive statistics. Pearson Chi-square test was used in order to analyze the association between the proportion of different bacteria in the gut and the variables used in the study. P less than =0.05 was held significant. Our research conducted 40 samples, 30 on each side were positive and 10 were negative. Out of 30 positive isolate 28 were targeted organism’s bacterium and the rest 2 were gram positive bacteria. There were a total of 8 (28.57%), 7 (25%) and 13 (46.42%) bee samples that showed the presence of K. pneumonia, E. coli and Serratia marcescenin, respectively. The isolated bacterial species exhibited a unique impact in a different research area. The analyzed hives contained 18 (60%) and 12 (40%) of the bacterial species present in traditional and contemporary hives, respectively. The study examined the influence of study region and hive type as potential risk factors; however, no statistically significant differences were observed in the number of identified bacterial species (P > 0.05).  Six commonly used antibiotics were employed against the bacterial isolates. These medications were evaluated against the isolates utilizing the disc diffusion technique. The tested medications included amoxicillin, ampicillin, tetracycline, vancomycin, gentamicin, and kanamycin. The susceptibility pattern tests revealed that all identified bacterial species exhibited high resistance to ampicillin, amoxicillin, tetracycline, and vancomycin, while demonstrating significant sensitivity to gentamicin and kanamycin. In this study, the pathogenic bacterial species were identified using the gram stain, morphology, and biochemical test. It will need careful observation and experimentation to determine the best method for removing pathogenic bacteria from honey bee guts.